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citT [2017-12-15 17:04:33]
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citT [2017-12-15 17:04:33]

two-component response regulator, regulation of citrate uptake
locus
BSU07590
pI
6.36
mw
25.26 kDa
protein length
226 aa Sequence Blast
gene length
678 bp Sequence Blast
function
regulation of citrate uptake
product
two-component response regulator
essential
no
synonyms
yflQ
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 2|Metabolism] → [category|SW 2.2|Carbon metabolism] → [category|SW 2.2.2|Utilization of specific carbon sources] → [category|SW 2.2.2.1|Utilization of organic acids]
  • [category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.2|Transcription factors and their control] → [category|SW 3.4.2.1|Two-component system response regulators]
  • [category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.2|Phosphorylation on an Asp residue]

    • Gene

    Coordinates
    832,545 → 833,225

    Phenotypes of a mutant

  • no growth with citrate as sole carbon source [Pubmed|10972810]

    • The protein

    Catalyzed reaction/ biological activity

  • binding to the promoter of the ''[gene|C0DED68802EF78D2BC16507234AAC3B60D236E68|citM]-[gene|66B9809A77AB15E5C8C8E5A8E26752771AF55E3A|yflN]'' operon and activation of transcription in the presence of citrate [Pubmed|10972810]

    • Modification

  • phosphorylated by [protein|7EBB09F8FF4C2FBE35B4689B15D89FEF3D58A227|CitS] on an Asp residue

    • Effectors of protein activity

  • phosphorylation likely affects DNA-binding activity

    • [SW|Localization]

  • cytoplasm (according to Swiss-Prot)

    • Expression and Regulation

    Operons

    genes
    [gene|7EBB09F8FF4C2FBE35B4689B15D89FEF3D58A227|citS]-[gene|1345FBA88CB55E0E897FEC8DA4D829B85A8393BB|citT]-[gene|E4F1B49C26BCB4E3E596406A83CCDCC20AD4A3DB|yflP]
    description
    [Pubmed|10972810]
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    Biological materials

    Mutant

  • MGNA-C250 (citT::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2248 NBRP B. subtilis, Japan]
  • BKE07590 (Δ[gene|1345FBA88CB55E0E897FEC8DA4D829B85A8393BB|citT]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE07590 BGSC], [Pubmed|28189581], upstream reverse: _UP1_AATCATCCTCCGCAATCGCG, downstream forward: _UP4_TATTATTTGGCGGCGGATTA
  • BKK07590 (Δ[gene|1345FBA88CB55E0E897FEC8DA4D829B85A8393BB|citT]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK07590 BGSC], [Pubmed|28189581], upstream reverse: _UP1_AATCATCCTCCGCAATCGCG, downstream forward: _UP4_TATTATTTGGCGGCGGATTA

    • References

    Reviews

  • 28152228

    • Original publications

  • 10094672,16842348,10972810